Efficacy study with Tetrahydrocurcuminoids

Efficacy of THP in enhancing the free radical scavenging effects of Tetrahydrocurcuminoids
  • Tetrahydrocurcuminoids (THC) is a naturally occurring polyphenol derived from turmeric root (Curcuma longa Linn.)
  • In the in vitro DPPH radical scavenging method, the ability of an antioxidant to bind and inactivate the 1,1 diphenyl-2-picrylhydrazyl radical or DPPH is measured
  • The DPPH is considered as an example of very stable free radical
  • The control sample contained 0.01% of THC and the active sample contained 0.01% of THC with 0.1% – 0.0001% concentrations of Tetrahydropiperine
  • Additionally the control containing various concentrations of Tetrahydropiperine was also tested for DPPH binding
Results
  • While THP by itself did not show any significant antioxidant properties, it was shown to enhance the antioxidant properties of THC by 19.3 – 61.8% as compared with THC when used alone
  • Even in its highest dilution of 0.0001% Tetrahydropiperine augmented the bioenhancing activity of THC to some extent
Enhancement of antioxidant properties of Tetrahydrocurcumin (THC) in presence of THP
A Conc (mg/mL) 1 x 10-2 1 x 10-3 1 x 10-3
%Inhibition 55.5 10.2 1.0
B Conc (mg/mL) 1 x 10-2 1 x 10-3 1 x 10-3
%Inhibition 8.1 3.3 3.2
C Conc (mg/mL) 1 x 10-2 for each 1 x 10-3 for each 1 x 10-3 for each
%Inhibition 66.2 16.5 5.8

Sample A: Tetrahydrocurcumin (THC)

Sample B: THP

Sample C: 1:1 mixture of Sample A and Sample B
DPPH was used at 100 μM conc. and optical density was measured at 517 nm